Tobacco Methods

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Moisture Content


Trace Metals



Analyte(s) Product Type Instrumentation References

Nicotine/Nicotine Alkaloids

All Tobacco Products

Liquid Chromatography – Tandem
Mass Spectrometry

“Simultaneous Determination of Tobacco Alkaloids, Tobacco-Specific Nitrosamines, and Solanesol in Consumer Products Using UPLC–ESI-MS/MS”. John R. Shifflett, Leah Watson, Devin J. McNally, and Dawit Z. Bezabeh, Chromatographia (2018), 81(3), 517-523.

Total Reducing Sugars (Sucrose, Fructose, and Glucose)

Pipe tobacco

Ion Chromatography

1, 2

Sieve Test

Chewing tobacco

Electric 8-inch sieve shaker, using horizontal circular sieving motion.  Testing sieves, covered with wire screens according to DIN ISO 3310 and A.S.T.M. E–11–87, No. 10 mesh (0.0787 inch or 2.00 millimeters), with covers and collecting pans.

TTB Procedure Number 2014–1, Testing of Chewing Tobacco and Snuff Products, January 14, 2014.

Product weight, pipe tobacco and RYO (roll your own)


Analytical balance


  1. Zook, C.M., Patel, P.M., LaCourse, W.R., Ralapati, S, (1996) Characterization of Tobacco Products by High-Performance Anion Exchange Chromatography-Pulsed Amperometric Detection, J. Agric. Food Chem., Vol 44, No. 7
  2. Dionex Technical Note 20, Analysis of Carbohydrates by High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection (2000),

Tobacco Links:

CORESTA - An association founded in 1956, ruled by French law, the purpose being to promote international cooperation in scientific research relative to tobacco.





Page last reviewed/updated: 06/10/2021